Linearity was determined by taking two serum samples treated with Dissociation Reagent and diluted 1:50 with Assay Buffer, one with a low diluted corticosterone level of 104.6pg/mL and one with a higher diluted level of 2,890.5pg/mL, and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.

Chemical structure of Corticosterone